Insertion junction: LMJ.RY0402.066297_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066297
Chromosome:chromosome 10
Location:480256
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g421021 (1 of 25) IPR001005//IPR009057//IPR017930 - SANT/Myb domain // Homeodomain-like // Myb domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCTGCTGCGCCTGCGCCTGCTGGTGTT
Internal bar code:AGGCTTCTAATCTAACGCTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:150
LEAP-Seq percent confirming:67.0051
LEAP-Seq n confirming:132
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGCTGATGAGTATCCCAC
Suggested primer 2:AGCAGTGCTCTCTACTCGGG