| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066317 |
| Chromosome: | chromosome_6 |
| Location: | 7511001 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g300500 | CYA18,ADCY1 | Adenylyl cyclase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAGAACGGCGGTGAGCGCATGCCGGCATG |
| Internal bar code: | GGCCGCCCGTGTTATAGATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.4737 |
| LEAP-Seq n confirming: | 1890 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTCTCCTTACCCGCTTT |
| Suggested primer 2: | ACAACGAGTTCATTACCGCC |