| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066386 |
| Chromosome: | chromosome 16 |
| Location: | 3600773 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683437 | (1 of 1) K16278 - E3 ubiquitin-protein ligase HOS1 [EC:6.3.2.19] (HOS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAACCCAAGATGAAACTGCACCCCACCA |
| Internal bar code: | GGACGGTAGGATGATATCAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 98.6573 |
| LEAP-Seq n confirming: | 9038 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCGTCAAAGATGCACTG |
| Suggested primer 2: | ACCAAGCGCTACAAGCCTAA |