| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066396 |
| Chromosome: | chromosome 3 |
| Location: | 1222719 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149700 | (1 of 1) PF14518 - Iron-containing redox enzyme (Haem_oxygenas_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGTGTGTGTGTGTGTCTTGGACAGA |
| Internal bar code: | GAGGGGGTATGTCCTTTTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATGGGTTCTCTCGATTC |
| Suggested primer 2: | TTTACGTTCCTCAAAACCCG |