Insertion junction: LMJ.RY0402.066403_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066403
Chromosome:chromosome 17
Location:4900035
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g734400 CUL2,CUL3 (1 of 1) K03347 - cullin 1 (CUL1, CDC53); Ubiquitin ligase SCF complex subunit Cullin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCTTTGTCGCTCACGTACGCCAGCAGCT
Internal bar code:CGGGTTGGCGATCGTCACACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:258
LEAP-Seq percent confirming:99.1087
LEAP-Seq n confirming:1112
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTGCTGCTGCTTCTCCT
Suggested primer 2:TACGTGTCCACCTGCTTCTG