| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066405 |
| Chromosome: | chromosome 6 |
| Location: | 5104825 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281000 | (1 of 52) PF00856 - SET domain (SET) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGGCACTGTTCGTGCTGGCTGTAGC |
| Internal bar code: | TCAGACGTAGGATTGGCGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 68.599 |
| LEAP-Seq n confirming: | 852 |
| LEAP-Seq n nonconfirming: | 390 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCAAGTCCGCAAGTGAC |
| Suggested primer 2: | CTAGACAGGCGCAACATTGA |