| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066416 |
| Chromosome: | chromosome 3 |
| Location: | 6586264 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196200 | (1 of 2) PTHR17630//PTHR17630:SF32 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCACCGGAGGGCGCGCCACAAACATGCG |
| Internal bar code: | AAACGGATCATGGTAGAAACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 998 |
| LEAP-Seq percent confirming: | 99.7782 |
| LEAP-Seq n confirming: | 4498 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAGGTGCAGGGTTTAGG |
| Suggested primer 2: | GAAGAGTGCTGGGGAGTGAG |