| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066436 |
| Chromosome: | chromosome 16 |
| Location: | 2314519 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659400 | (1 of 1) PTHR23257:SF347 - SERINE/THREONINE-PROTEIN KINASE KINX-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCTGCTCCGGCACGCGCCTGCAGCCCA |
| Internal bar code: | GTTGGTAAGTGAGATGCAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 882 |
| LEAP-Seq percent confirming: | 98.8464 |
| LEAP-Seq n confirming: | 2999 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCAGCTTCGGCATAGTG |
| Suggested primer 2: | CTTGCCATACCACCCATACC |