Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.066440 |
Chromosome: | chromosome 10 |
Location: | 5040762 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g455700 | PAP5 | Non-canonical poly(A) polymerase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACGACGCCTACAAGTGGGTGCTTCCTAG |
Internal bar code: | GACTCAACTATCGCGCTCTCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 181 |
LEAP-Seq percent confirming: | 83.3333 |
LEAP-Seq n confirming: | 5 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCAGCCCAAACTGGTAGG |
Suggested primer 2: | CTCCTGCCTCTGCTACTGCT |