Insertion junction: LMJ.RY0402.066471_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066471
Chromosome:chromosome 3
Location:2690692
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g161250 CYP748A1,CYP14 (1 of 2) PTHR24305:SF65 - CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTACGCAGGCACGCCGCCGCAACCCCCAG
Internal bar code:AAGTGGAACGGGGGCTGATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:331
LEAP-Seq percent confirming:52.9412
LEAP-Seq n confirming:414
LEAP-Seq n nonconfirming:368
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGACATCCAGGAACACT
Suggested primer 2:TTGGTGGCTGGATAAGGAAG