| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.066475 |
| Chromosome: | chromosome 7 |
| Location: | 2069671 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325950 | ZSP1 | (1 of 2) PTHR10783:SF44 - SPX DOMAIN-CONTAINING PROTEIN 1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGTTGTCTCGGCCAGCTGCCGCCCTGC |
| Internal bar code: | AAATCATAGGATAGGTGGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 92.5911 |
| LEAP-Seq n confirming: | 8948 |
| LEAP-Seq n nonconfirming: | 716 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCGTTCATCAATACCCT |
| Suggested primer 2: | TTGGACATTATGGCAGTGGA |