Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.066491 |
Chromosome: | chromosome_17 |
Location: | 1141347 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g704300 | FAP47 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGCGTCACGCTGAAGTCGCTGCGCGCCCT |
Internal bar code: | ACTGGCTACCGATGACGGAGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 252 |
LEAP-Seq percent confirming: | 88.5231 |
LEAP-Seq n confirming: | 995 |
LEAP-Seq n nonconfirming: | 129 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTAAGCAGATGCTGGGTTC |
Suggested primer 2: | TGATCTTGAGCACCATCTCG |