| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066500 |
| Chromosome: | chromosome 16 |
| Location: | 4920926 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGGCTGGCGGCGGAGGAGCGCGAGAGC |
| Internal bar code: | ATTGCGCGCGTCGCTTGTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 17 |
| LEAP-Seq percent confirming: | 58.8589 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGCTGGGTCTTGGTTTA |
| Suggested primer 2: | AGGAGGCGCGTAGACATAGA |