Insertion junction: LMJ.RY0402.066546_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.066546
Chromosome:chromosome 14
Location:3822951
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g632700 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACTGCTGCCTTGCGTTCCCGTCTGCGCA
Internal bar code:ATAATCAATTGGGTGTCGCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:557
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCACTGGTTGGGCTCTCT
Suggested primer 2:GGTGCATCTAGCCTTCAAGC