| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.066549 |
| Chromosome: | chromosome 16 |
| Location: | 828424 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647602 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAACCCCACACACACGCTCAGCCCCACC |
| Internal bar code: | CATGCCTCCTATGCGTTTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 31 |
| LEAP-Seq percent confirming: | 70.9205 |
| LEAP-Seq n confirming: | 2034 |
| LEAP-Seq n nonconfirming: | 834 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGATCGGAGTGAGGACGC |
| Suggested primer 2: | CTTCCTCCTTCGAGATGCAC |