| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066558 |
| Chromosome: | chromosome 2 |
| Location: | 6877003 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119651 | (1 of 4) 2.4.1.229 - [Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase / transferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGCGCGAGTGCTGGCGGTGCTGCGTAC |
| Internal bar code: | GTAAAATTTAAGGCGGCGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 95.1456 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTCCACTCTAGAGGCCG |
| Suggested primer 2: | TGTGTCAGAGAGCATCAGGG |