| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066558 |
| Chromosome: | chromosome 10 |
| Location: | 1362725 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427850 | (1 of 1) IPR000014//IPR000104//IPR013767 - PAS domain // Antifreeze protein, type I // PAS fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGTCGTCTTGAGCCCGGGCGCTGCTGA |
| Internal bar code: | GAGAGGGGGGCGCGTATAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 57.8843 |
| LEAP-Seq n confirming: | 2632 |
| LEAP-Seq n nonconfirming: | 1915 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGACTTTCGTCGCTCCT |
| Suggested primer 2: | GTGAGAGTGCGAGGAAGGAC |