Insertion junction: LMJ.RY0402.066564_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.066564
Chromosome:chromosome 6
Location:5247229
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g282300 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCAATTAGCGCGTGGTGTGACAGTTCA
Internal bar code:GTGGGCAGGATGGATTGAGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:39
LEAP-Seq percent confirming:12.4183
LEAP-Seq n confirming:133
LEAP-Seq n nonconfirming:938
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTGGTACATTGGAGGGTT
Suggested primer 2:CTCCTTTGACGAAGTCTGCC