| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066609 |
| Chromosome: | chromosome 2 |
| Location: | 1562821 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084800 | SPO11A | (1 of 1) K10878 - meiotic recombination protein SPO11 (SPO11); meoisis-related DNA topoisomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCAGTCGCAGTCGGACGCCATTTTGGA |
| Internal bar code: | ATCCGTTGCCGAGGTGTGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 60.8079 |
| LEAP-Seq n confirming: | 1114 |
| LEAP-Seq n nonconfirming: | 718 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGGACCTTCCCTACCA |
| Suggested primer 2: | GAGTGGCTCATCTCCTCCTG |