Insertion junction: LMJ.RY0402.066619_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066619
Chromosome:chromosome 6
Location:5840743
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g287800 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAAACTCCCTGTGTGCCCCCAGCACGCC
Internal bar code:CGGACTTGGGTGTCGTCGCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:99.469
LEAP-Seq n confirming:10678
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GAACATCAGATGCGTTCCCT
Suggested primer 2:GCCTACGGTTCTCACTGCTC