| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066627 |
| Chromosome: | chromosome 17 |
| Location: | 665198 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700650 | GFY2 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCGAACCCAGCCTTGCAGGTCTAGGCC |
| Internal bar code: | GCATGGGACGTCAACTCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 98.7379 |
| LEAP-Seq n confirming: | 6415 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAACATTGCTTTGTTGG |
| Suggested primer 2: | CTATTTATGCCGGTGCCAGT |