Insertion junction: LMJ.RY0402.066628_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066628
Chromosome:chromosome 6
Location:8710511
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g309800 MOT57,MOT2 Predicted protein in motile organisms; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGAAGGTCCTCCCCTGCTTCTGTCATAT
Internal bar code:CACACCAGCGCGGCTGGGCCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:193
LEAP-Seq percent confirming:45.611
LEAP-Seq n confirming:1829
LEAP-Seq n nonconfirming:2181
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTAGACAAGTGTTCCCCA
Suggested primer 2:GAACGAGGACTGCTGTGTCA