| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066675 |
| Chromosome: | chromosome 12 |
| Location: | 7655426 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554550 | (1 of 1) IPR026771 - Transmembrane protein 218 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCATCGGGTCACGGACCAAGTTCAGAT |
| Internal bar code: | TGGGGCCCGGAAGAGTCAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 87 |
| LEAP-Seq percent confirming: | 73.2446 |
| LEAP-Seq n confirming: | 1982 |
| LEAP-Seq n nonconfirming: | 724 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCAAGCTGTCCAGTCAC |
| Suggested primer 2: | ATGGTCTGGTTGTAGCCCTG |