| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.066675 |
| Chromosome: | chromosome 13 |
| Location: | 1690325 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574200 | PAP2 | (1 of 3) K03514 - non-canonical poly(A) RNA polymerase PAPD5/7 (PAPD5_7, TRF4); Class-II RNA nucleotidyl transferase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCCTCGTGCTTTTCTATGCGCCACCGC |
| Internal bar code: | TGGGCCTAACCGGATTTCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 99.4312 |
| LEAP-Seq n confirming: | 6293 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAACAGGTCATCCTCTGG |
| Suggested primer 2: | ATAACACCGGTCTCACCTGC |