Insertion junction: LMJ.RY0402.066710_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.066710
Chromosome:chromosome 9
Location:2480647
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g391150 DIV153 (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCAGTGACCCGCACTCCAACTACATGA
Internal bar code:TCAAGCCCGGTGGAGTGAGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:256
LEAP-Seq percent confirming:98.3222
LEAP-Seq n confirming:1758
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCTTACATGTGCTTGCCG
Suggested primer 2:CCGATTCGTGTGGCTAGATT