| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066736 |
| Chromosome: | chromosome 17 |
| Location: | 706179 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700950 | FDX5 | (1 of 2) PTHR19370//PTHR19370:SF110 - NADH-CYTOCHROME B5 REDUCTASE // SUBFAMILY NOT NAMED; Ferredoxin 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTGTTCGACGCCGTGGAGCGCTACGAT |
| Internal bar code: | CCATCAATTGTCAGGCCCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 99.6689 |
| LEAP-Seq n confirming: | 301 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAAATCAGCGCAGGAGT |
| Suggested primer 2: | GAAGCTTCATCAGCGAATCC |