Insertion junction: LMJ.RY0402.066748_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066748
Chromosome:chromosome 9
Location:3434684
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g389208 (1 of 2) PTHR11266//PTHR11266:SF17 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // PROTEIN MPV17 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCAGACCAGCTTCCCCCAGCCGCGCCGCT
Internal bar code:GCCGCGCCGGCTGATTCCCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:754
LEAP-Seq percent confirming:94.7521
LEAP-Seq n confirming:4261
LEAP-Seq n nonconfirming:236
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGATGTTTTGGCTGCTT
Suggested primer 2:GTCTCTCTCCCAAGACGTGC