| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066766 |
| Chromosome: | chromosome_1 |
| Location: | 5533661 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g039200 | IFT22,FAP9 | Intraflagellar transport protein 22 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCCGCACAGGCTCCGTATCTACCGTATGT |
| Internal bar code: | GGTAGGATTGCGACGAACCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 55.7143 |
| LEAP-Seq n confirming: | 546 |
| LEAP-Seq n nonconfirming: | 434 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGATTCACGAGTTGCCAG |
| Suggested primer 2: | CGATGTGTTCACCCTGTACG |