| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066767 |
| Chromosome: | chromosome_17 |
| Location: | 5455789 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g737100 | FAP127 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGCCGCTTCGCTTGCCAACAGCCTTGCGG |
| Internal bar code: | TTATGGAATCACATAGTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 85.3339 |
| LEAP-Seq n confirming: | 10159 |
| LEAP-Seq n nonconfirming: | 1746 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTGGATTGGAGTGATG |
| Suggested primer 2: | AGGCGTATAGTTGGCATTGG |