| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.066775 |
| Chromosome: | chromosome 6 |
| Location: | 3280862 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277050 | (1 of 1) K14856 - protein SDA1 (SDA1, SDAD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCGCTCGCCGCCGCCGCTCAGCCGCCC |
| Internal bar code: | CGTGAGCGTAGGGCGCCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 23.2919 |
| LEAP-Seq n confirming: | 150 |
| LEAP-Seq n nonconfirming: | 494 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCTGGGGTATGGGGTTT |
| Suggested primer 2: | CTTCACGCTGTTCTCCATGA |