| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066777 |
| Chromosome: | chromosome 10 |
| Location: | 3456675 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACCCATGTACAGTTACTACCAATGAAA |
| Internal bar code: | GGTGGTGACGCCGAGTTGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 99.3634 |
| LEAP-Seq n confirming: | 3434 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGCTGCCAGACCAGACC |
| Suggested primer 2: | CTATGTTAGCAAGCGGGAGC |