Insertion junction: LMJ.RY0402.066781_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.066781
Chromosome:chromosome 13
Location:1318900
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g571251 (1 of 8) IPR001163//IPR010920 - LSM domain, eukaryotic/archaea-type // LSM domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACCCGCTGAGGGCGCTCCACACGCCGGG
Internal bar code:GGCTCGGGACGCACGGTGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:470
LEAP-Seq percent confirming:99.65
LEAP-Seq n confirming:1993
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GACCACACAAGGGTTTGCTT
Suggested primer 2:AAAGGGCAACTGCACCATAC