| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066787 |
| Chromosome: | chromosome 5 |
| Location: | 1775292 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242350 | Putative calcium-transporting ATPase, endoplasmic reticulum-type; (1 of 1) PTHR24093:SF289 - CALCIUM-TRANSPORTING ATPASE 1, ENDOPLASMIC RETICULUM-TYPE-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGACGATGACACGCCGCTCAAGAAGA |
| Internal bar code: | AGGCGCTCGGGCCGGGATGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 62.8763 |
| LEAP-Seq n confirming: | 376 |
| LEAP-Seq n nonconfirming: | 222 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGTGTACAACCCTCGCC |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |