| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066822 |
| Chromosome: | chromosome 8 |
| Location: | 2481508 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g371801 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGAGACTGGGAAACACTTGTCCCCAAG |
| Internal bar code: | CCTCCCACGGCGGCACCGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 88.6511 |
| LEAP-Seq n confirming: | 2320 |
| LEAP-Seq n nonconfirming: | 297 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATCAACTCTGCCCTCGCT |
| Suggested primer 2: | CCGGCCAACCTAACAACTAA |