Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.066824 |
Chromosome: | chromosome_9 |
Location: | 7701100 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g415650 | antisense | 5'UTR_intron|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCTTAACGGAACAAGGGCTTGCAGTTCCC |
Internal bar code: | GTCCTCCTTCGAAAGGTGCAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 966 |
LEAP-Seq percent confirming: | 99.4379 |
LEAP-Seq n confirming: | 3184 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGTGGTTGACGGCAAAAAT |
Suggested primer 2: | CTCAAAAGGCAGAGGGACTG |