| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066829 |
| Chromosome: | scaffold 18 |
| Location: | 229091 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749797 | FAP281 | Flagellar Associated Coiled-Coil Protein 281 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGGGGCGCTGCTGGCGGCGCAGGAGC |
| Internal bar code: | CGGATTAGCTTTTCTTGGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 26.6221 |
| LEAP-Seq n confirming: | 279 |
| LEAP-Seq n nonconfirming: | 769 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACCATTGCAGGACATTG |
| Suggested primer 2: | TACCAGCCCAAACTAAACCG |