| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066830 |
| Chromosome: | chromosome_9 |
| Location: | 658207 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g403200 | FAP198 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCGGGCATGACAGACCTGGGCATGGGGG |
| Internal bar code: | CAGGGCTTACCCAAGGCAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 56.2977 |
| LEAP-Seq n confirming: | 295 |
| LEAP-Seq n nonconfirming: | 229 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATGCTCATCCATTCTCT |
| Suggested primer 2: | GGGAGGTGTGAAGAACCAGA |