| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066833 |
| Chromosome: | chromosome 3 |
| Location: | 7888380 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCACACGTTTGCCAAACTCGACGCCCC |
| Internal bar code: | GAGTCCCCTCGGAGGGGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 78.529 |
| LEAP-Seq n confirming: | 1057 |
| LEAP-Seq n nonconfirming: | 289 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCGATACCGGATATAA |
| Suggested primer 2: | GTACTCACAGCGCAGGTTGA |