| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.066858 |
| Chromosome: | chromosome 3 |
| Location: | 1785411 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154150 | (1 of 1) K12489 - Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein (ACAP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGTGGAGGAGGCAGCCCGTTGCAAGGC |
| Internal bar code: | ACCTATCGTGGGATGCGAACTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 94.2503 |
| LEAP-Seq n confirming: | 5098 |
| LEAP-Seq n nonconfirming: | 311 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCCTCAATGTTGTTAGT |
| Suggested primer 2: | AGAGAGCCGAACTAGCCACA |