Insertion junction: LMJ.RY0402.066879_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.066879
Chromosome:chromosome 17
Location:2836093
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g719250 (1 of 5) K16302 - metal transporter CNNM (CNNM) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAGCGTGTCCACTACTGTCACCTGGTGT
Internal bar code:CTTCGCTACTCTCAGTAAAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:7
LEAP-Seq percent confirming:99.1157
LEAP-Seq n confirming:6949
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCTTCCGAGCAAACGTC
Suggested primer 2:CCTCATCCGTGTCAATCCTT