| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.066910 |
| Chromosome: | chromosome 7 |
| Location: | 4258303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341850 | TIF2,INFB,IF2,EIF2,CIF2 | Chloroplast translation initiation factor 2; (1 of 1) PTHR23115//PTHR23115:SF138 - TRANSLATION FACTOR // TRANSLATION INITIATION FACTOR IF-2, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCCATGGGCTGTGATTCCCGCTGTACC |
| Internal bar code: | CGGCTGCTTAGCTTAACGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGAGGACAAGCTCAAGGT |
| Suggested primer 2: | GGTGGAAAGCCCTAACACAA |