| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.066975 |
| Chromosome: | chromosome 10 |
| Location: | 6002901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462816 | (1 of 2) IPR007112//IPR007117//IPR009009 - Expansin/pollen allergen, DPBB domain // Expansin, cellulose-binding-like domain // RlpA-like double-psi beta-barrel domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGGCAAGTTGGGTGGGTGTGTTGGTGCC |
| Internal bar code: | TGTACGGTACCGAATTTGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 99.6951 |
| LEAP-Seq n confirming: | 327 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCCACAGTCAAGGAAT |
| Suggested primer 2: | GTTGAAGGAGGCAATTTGGA |