| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.066975 |
| Chromosome: | chromosome 16 |
| Location: | 5875504 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677450 | (1 of 2) 5.1.3.15 - Glucose-6-phosphate 1-epimerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGAGCGCCCATGAGCTGAACCTGCCAA |
| Internal bar code: | ATGTGCTAACTTGACCTGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1266 |
| LEAP-Seq percent confirming: | 99.0683 |
| LEAP-Seq n confirming: | 2233 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAACTACCCGAAGCCCAC |
| Suggested primer 2: | AAGATGTGGAACGGATGAGC |