| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.067053 |
| Chromosome: | chromosome 13 |
| Location: | 989243 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568350 | BLZ18 | bZIP transcription factor | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGTTTCACGAGGAGCAAGACACTTGGC |
| Internal bar code: | AGGCGAACAGCGTCGAAGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 79.0765 |
| LEAP-Seq n confirming: | 1096 |
| LEAP-Seq n nonconfirming: | 290 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCCTACTGCTTCCTGCTG |
| Suggested primer 2: | CGTCTCAGACATAGCCGTCA |