| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.067065 |
| Chromosome: | chromosome 3 |
| Location: | 1883260 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154950 | GST6 | Glutathione S-transferase; (1 of 3) K07393 - putative glutathione S-transferase (ECM4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCCGTGTCGGGCCTGATGGCGGTCCTT |
| Internal bar code: | AATTTGAATCTTTTTGGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 99.6063 |
| LEAP-Seq n confirming: | 506 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGTCAACTGCGTGTGAG |
| Suggested primer 2: | AACCACAGAGACATGAGGGG |