| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.067079 |
| Chromosome: | chromosome 13 |
| Location: | 983551 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568350 | BLZ18 | bZIP transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGCAGTTATATGCAGTCGTAGCAATCG |
| Internal bar code: | TCCGACCCTTGTTGTCGCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 96.4085 |
| LEAP-Seq n confirming: | 4295 |
| LEAP-Seq n nonconfirming: | 160 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGATGCAGTGCAAGTT |
| Suggested primer 2: | GGCTGCAAGCAGACATACAA |