| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.067167 |
| Chromosome: | chromosome 6 |
| Location: | 2152418 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265800 | PRPL28 | Chloroplast ribosomal protein L28, imported to chloroplast; (1 of 1) PTHR13528//PTHR13528:SF5 - 39S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL // SUBFAMILY NOT NAMED | CDS/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACTCCGACAGGGTGATCTTCAGCTCCTG |
| Internal bar code: | CCTGCTCGGCGGAATCTCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 99.8221 |
| LEAP-Seq n confirming: | 2805 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCTCACGTTCTTCGTT |
| Suggested primer 2: | AAATTACACTGCGGGTGCTC |