Insertion junction: LMJ.RY0402.067187_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.067187
Chromosome:chromosome 12
Location:8020380
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTAGATGGAAGATACGAGCACTGTCCTC
Internal bar code:GAGGCAACACGCCAGCGGTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:326
LEAP-Seq percent confirming:56.8918
LEAP-Seq n confirming:615
LEAP-Seq n nonconfirming:466
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACACCATGCCACTCAACTC
Suggested primer 2:AGGAAACGACTACGAAGCGA