| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067189 |
| Chromosome: | chromosome 4 |
| Location: | 1597188 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213250 | (1 of 2) K01256 - aminopeptidase N (pepN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGAGAGGACTCACGCAGCATGCCACGTG |
| Internal bar code: | CCGCGAGCCTTTGAACACGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 85.1429 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGTTCGGTTTCAGTTTG |
| Suggested primer 2: | ACGATTATGAAGGACGGTGC |