| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067190 |
| Chromosome: | chromosome 11 |
| Location: | 3667842 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482483 | (1 of 1) K12599 - antiviral helicase SKI2 (SKI2, SKIV2L) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGTGTGTGTGTGTGTGTTGTGCCGC |
| Internal bar code: | GATGGGTATCTTTGACGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 54.491 |
| LEAP-Seq n confirming: | 176436 |
| LEAP-Seq n nonconfirming: | 147353 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCCTTGCAGAGCAAAAA |
| Suggested primer 2: | AGCGTGCATGTTTCTGAATG |